血小板衍生生長因子A(PDGFA)重組蛋白
Recombinant Platelet Derived Growth Factor Subunit A (PDGFA)
PDGF-A; PDGF1; Platelet Derived Growth Factor Alpha Polypeptide
- 編號RPA528Ra01
- 物種Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 來源原核表達
- 宿主E.coli
- 內毒素水平<1.0EU/µg(LAL法測定)
- 亞細胞定位分泌
- 預測分子量15.2kDa
- 實際分子量15kDa(差異分析請參閱說明書)
- 片段與標簽Glu90~Glu190 with N-terminal His Tag
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點9.1
- 應用Positive Control; Immunogen; SDS-PAGE; WB.
如果需要有生物活性的蛋白，請參見活性蛋白。 - 下載英文說明書 中文說明書
- 規(guī)格10μg50μg200μg1mg5mg
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產品包裝(模擬)
產品包裝(模擬)
Figure . Gene Sequencing (extract)
Figure. SDS-PAGE
通過ISO 9001、ISO 13485質量體系認證
序列
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
增值服務
相關產品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA528Ra01 | 血小板衍生生長因子A(PDGFA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA528Ra61 | 血小板衍生生長因子A(PDGFA)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA528Ra01 | 血小板衍生生長因子A(PDGFA)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA528Ra | 血小板衍生生長因子A(PDGFA)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA528Ra | 血小板衍生生長因子A(PDGFA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Canadian Journal of Physiology and Pharmacology | The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats[pubmed:28177680] |
| International Immunopharmacology | Shikonin alleviates allergic airway remodeling by inhibiting the ERK-NF-κB signaling pathway[pubmed:28521243] |
| Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells[] |
| Prostaglandins & other Lipid Mediators | Lipoxin A4 inhibited the activation of Hepatic Stellate Cells-T6 cells by modulating profibrotic cytokines and NF-κB signaling pathway[Pubmed: 31698141] |
| Ann Agric Environ Med | PDGF-BB homodimer serum level–a good indicator of the severity of alcoholic liver cirrhosis[Pubmed: 32208584] |
| Tissue Eng Regen Med | Effect of Leukocyte-Platelet Rich Fibrin (L-PRF) on Tissue Regeneration and Proliferation of Human Gingival Fibroblast Cells Cultured Using a Modified Method[34339025] |
| Biomed Research International | Thermal Oscillation Changes the Liquid-Form Autologous Platelet-Rich Plasma into Paste-Like Form[Pubmed:35586817] |