血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)
Multiplex Assay Kit for Vanin 1 (VNN1) ,etc. by CBA (Cytometric Bead Array)
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-97 | 94 |
| EDTA plasma(n=5) | 80-98 | 90 |
| heparin plasma(n=5) | 78-105 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-102% | 79-89% | 95-102% | 82-92% |
| EDTA plasma(n=5) | 79-102% | 89-96% | 79-97% | 95-102% |
| heparin plasma(n=5) | 80-92% | 94-103% | 98-105% | 91-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1、向實驗板每孔內加入200μL分析緩沖液以預濕；
2、實驗前標準品、試劑及樣本準備；
3、加樣（標準品、樣本、磁珠），標準品或樣本100μL及磁珠10μL，25°C酶標板振蕩器孵育2小時；
4、磁吸甩干，加檢測溶液A 100μL，25°C酶標板振蕩器孵育1小時；
5、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
6、磁吸洗板1次；
7、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實驗原理
將血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)抗體包被于磁性微球表面，制成固相載體，向微孔中分別加入標準品或標本以及磁珠，其中的血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)與連接于固相載體上的抗體結合，然后加入生物素化的血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)抗體，加入PE標記的親和素，用流式細胞儀讀數(shù)。MFI值和樣品中的血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法)濃度呈正相關。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPC598Ra01 | 血管非炎性蛋白1(VNN1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC598Ra01 | 血管非炎性蛋白1(VNN1)多克隆抗體 | WB |
| MAC598Ra21 | 血管非炎性蛋白1(VNN1)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC598Ra | 血管非炎性蛋白1(VNN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CMC598Ra | 血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光法) | FLIA Kit for Antigen Detection. |
| LMC598Ra | 血管非炎性蛋白1(VNN1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Toxicology | Vanin-1; a potential biomarker for nephrotoxicant-induced renal injury[ScienceDirect: S0300483X11003234] |
| Journal of Pharmacology and Experimental Therapeutics | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury[Aspet: 192807] |
| J Pharmacol Exp Ther | Urinary vanin-1 as a novel biomarker for early detection of drug-induced acute kidney injury.[PubMed: 22399813] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis[PubMed: 23307618] |
| Iranian Journal of Health and Physical Activity | Effects of Exercise and Ferula Gummosa on Apelin of Cardiac and Kidney Tissues in L-NAME induced Hypertension in Rats[Jm.um: Source] |
| Platelets | Value of vanin-1 assessment in adult patients with primary immune thrombocytopenia[Pubmed: 23534352] |
| Journal of Applied Toxicology | Early detection of renal injury using urinary vanin-1 in rats with experimental colitis.[Pubmed: 23307618] |
| Diabetes & Vascular Disease Research | Urinary Kim-1 is a sensitive biomarker for the early stage of diabetic nephropathy in Otsuka Long-Evans Tokushima Fatty rats[Sagepub:Source] |
| Food Chem Toxicol. | The effect of activated charcoal on adenine-induced chronic renal failure in rats[Pubmed:24412558] |
| Hypertension Research | Early urinary biomarkers for renal tubular damage in spontaneously hypertensive rats on a high salt intake[PubMed: 26376947] |
| Inflammation | Citrate Attenuates Adenine-Induced Chronic Renal Failure in Rats by Modulating the Th17/Treg Cell Balance[PubMed: 26253296] |
| Toxicology | Early prediction of cisplatin-induced nephrotoxicity by urinary vanin-1 in patients with urothelial carcinoma[Pubmed:27317936] |
| J Biomed Mater Res A | Nanosized titanium dioxide resulted in the activation of TGF-β/Smads/p38MAPK pathway in renal inflammation and fibration of mice[Pubmed:26850371] |
| APMIS | Citrate attenuates vascular calcification in chronic renal failure rats.[pubmed:28332248] |
| Clin Exp Pharmacol Physiol. | Early urinary biomarkers of renal tubular damage by a high-salt intake independent of blood pressure in normotensive rats.[pubmed:29027259] |
| Cellular?Physiology and Biochemistry | Gum Acacia Improves Renal Function and Ameliorates Systemic Inflammation, Oxidative and Nitrosative Stress in Streptozotocin-Induced Diabetes in Rats?…[Pubmed:29550811] |
| International Journal of Molecular?Sciences | Vanin-1 in Renal Pelvic Urine Reflects Kidney Injury in a Rat Model of Hydronephrosis[Pubmed: 30332759] |
| International Journal of Molecular Sciences | Involvement of Vanin-1 in Ameliorating Effect of Oxidative Renal Tubular Injury in Dahl-Salt Sensitive Rats[Pubmed: 31514290] |
| The Journal of Clinical Hypertension homepage | Urinary vanin‐1 associated with chronic kidney disease in hypertensive patients: A pilot study[Pubmed: 32869948] |
| J Clin Hypertens | Association of urinary vanin?\1 with kidney function decline in hypertensive patients[34029440] |
| Plasma vanin-1 as a novel biomarker of sepsis for trauma patients: a prospective multicenter cohort study[33624223] |