血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Angiotensin 1-7 (Ang1-7) ,etc. by FLIA (Flow Luminescence Immunoassay)
（注：單次混測多因子不超過8個指標(biāo) ）
- 編號LMS085Mi
- 物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時長3h
- 檢測范圍0.78-800pg/mL
- 靈敏度最小可檢測劑量小于等于0.26 pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載英文說明書 中文說明書
- 規(guī)格8指標(biāo)數(shù)7指標(biāo)數(shù)6指標(biāo)數(shù)5指標(biāo)數(shù)4指標(biāo)數(shù)3指標(biāo)數(shù)2指標(biāo)數(shù)1指標(biāo)數(shù)
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產(chǎn)品包裝(模擬)
產(chǎn)品包裝(模擬)
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒對檢測血管緊張素1-7(Ang1-7)具有較高的靈敏度。
經(jīng)檢測人、小鼠、大鼠之間有100%的交叉反映。
回收率
分別于定值血清及血漿樣本中加入一定量的血管緊張素1-7(Ang1-7)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-101 | 96 |
| EDTA plasma(n=5) | 78-94 | 80 |
| heparin plasma(n=5) | 84-96 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管緊張素1-7(Ang1-7)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管緊張素1-7(Ang1-7)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-99% | 89-103% | 88-105% | 95-103% |
| EDTA plasma(n=5) | 93-101% | 90-104% | 87-94% | 87-97% |
| heparin plasma(n=5) | 93-102% | 80-101% | 87-96% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1、向?qū)嶒?yàn)板每孔內(nèi)加入200μL分析緩沖液以預(yù)濕；
2、實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
3、加樣（標(biāo)準(zhǔn)品、樣本、磁珠、檢測溶液A），標(biāo)準(zhǔn)品或樣本50μL，加檢測溶液A 50μL，及磁珠10μL，25°C酶標(biāo)板振蕩器孵育90分鐘；
4、磁吸甩干，加檢測溶液B 100μL，25°C振動孵育30分鐘；
5、磁吸洗板1次；
6、加鞘液100μL，旋渦震蕩10分鐘后讀數(shù)。
實(shí)驗(yàn)原理
將血管緊張素1-7(Ang1-7)抗體包被于磁性微球表面，制成固相載體。在微孔中同時加入待測抗原（標(biāo)準(zhǔn)品或樣本）、生物素標(biāo)記的抗原和微球。待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。加入PE標(biāo)記的鏈霉親和素，上機(jī)讀數(shù)。MFI值（Median Fluorescence Intensity）和樣品中的血管緊張素1-7(Ang1-7)濃度呈負(fù)相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| CPS085Mi21 | 血管緊張素1-7(Ang1-7)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPS085Mi11 | 血管緊張素1-7(Ang1-7)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAS085Mi08 | 血管緊張素1-7(Ang1-7)多克隆抗體 | IHC |
| MAS085Mi27 | 血管緊張素1-7(Ang1-7)單克隆抗體 | WB; IHC; ICC; IP. |
| MAS085Mi22 | 血管緊張素1-7(Ang1-7)單克隆抗體 | IHC |
| MAS085Mi21 | 血管緊張素1-7(Ang1-7)單克隆抗體 | WB; IHC |
| MES085Mi | 血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CES085Mi | 血管緊張素1-7(Ang1-7)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMS085Mi | 血管緊張素1-7(Ang1-7)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| J Stroke Cerebrovasc Dis | Improvement of Plasma Biomarkers after Switching Stroke Patients from Other Angiotensin II Type I Receptor Blockers to Olmesartan[PubMed: 25891757] |
| Cellular Physiology and Biochemistry | Angiotensin-(1-7) in Paraventricular Nucleus Contributes to the Enhanced Cardiac Sympathetic Afferent Reflex and Sympathetic Activity in Chronic Heart Failure Rats[pubmed:28848201] |
| Frontiers in Physiology | Hydrogen Sulfide Attenuates Atherosclerosis in a Partially Ligated Carotid Artery Mouse model via Regulating Angiotensin Converting Enzyme 2 Expression[pubmed:29066981] |
| Molecular Medicine Reports | The ACE2-Ang (1-7)-Mas receptor axis attenuates cardiac remodeling and fibrosis in post-myocardial infarction.[pubmed:28656296] |
| Journal of Clinical Pharmacy and Therapeutics | Relationship between genetic variants of ACE2 gene and circulating levels of ACE2 and its metabolites.[pubmed:28895159] |
| Molecular Medicine Reports | Effects of IQP, VEP and Spirulina platensis hydrolysates on the local kidney renin angiotensin system in spontaneously hypertensive rats[pubmed:28944898] |
| Open Journal of Endocrine and Metabolic Diseases Paper Submission | A Study of the Correlation between Angiotensin (1-7) and the Histopathological Changes in the Penises of Experimentally Diabetic Rats[2-1980272_83198.htm] |
| Journal of the American Society of Hypertension | Mesenchymal stem cell–derived microvesicles alleviate pulmonary arterial hypertension by regulating renin-angiotensin system[Pubmed:29752040] |
| Innate?Immunity? | Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axis[Pubmed:29969931] |
| Evidence-based?Complementary and Alternative?Medicine? | Acute Biochemical, Cardiovascular, and Autonomic Response to Hyperbaric (4 atm) Exposure in Healthy Subjects[Pubmed:29977313] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | The impact of ovulation inducing drugs on carcinogenesis[] |
| European Journal of Obstetrics & Gynecology and Reproductive Biology | Changes of angiotensin II (1–8) and angiotensin (1–7) levels in early and late-onset preeclampsia[] |
| Journals of Gerontology Series A-Biological Sciences and Medical Sciences | Therapeutic Delivery of Ang (1-7) Via Genetically Modified Probiotic: A Dosing Study.[Pubmed: 31586210] |
| journal of the european academy of dermatology and venereology | Significance of the angiotensin I/angiotensin II/angiotensin‐(1‐7) axis in the pathogenesis of systemic sclerosis[Pubmed: 31746507] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study[Pubmed: 32756181] |
| Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?…[] | |
| Amino Acids | Angiotensin-(1¨C7) attenuates collagen-induced arthritis via inhibiting oxidative stress in rats[33398523] |
| Autoimmunity | The prevalence and role of functional autoantibodies to angiotensin-converting-enzyme-2 in patients with systemic sclerosis[33910447] |
| researchsquare | Ang-(1-7) Protects Skeletal Muscle Function in Aged Mice[] |
| Peptides | Angiotensin-(1-7) protects against sepsis-associated left ventricular dysfunction induced by lipopolysaccharide[34298021] |
| BMC Musculoskeletal Disorders | Ang-(1–7) protects skeletal muscle function in aged mice[34548056] |
| J Cardiovasc Pharmacol | Resveratrol Confers Protection Against Ischemia/Reperfusion Injury by Increase of Angiotensin (1-7) Expression in a Rat Model of Myocardial Hypertrophy[34232225] |